Course: Analytic chemistry

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Course title Analytic chemistry
Course code KAZ/ACH
Organizational form of instruction Lecture + Tutorial
Level of course Bachelor
Year of study not specified
Semester Winter
Number of ECTS credits 2
Language of instruction Czech
Status of course unspecified
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Course availability The course is available to visiting students
Lecturer(s)
  • Černý Radim, Prof. MUDr. CSc.
  • Vrzalová Jindra, RNDr. Ph.D.
  • Windrichová Jindra, RNDr. Ph.D.
Course content
Lectures: 1.General procedures in analytic chemistry. Harvest and preparation of samples for analysis. Preanalytic errors in laboratory medicine. 2.Types of analytic reactions. Quality parameters of analytical reactions. Selectivity and sensitivity of analytical reactions, imprecision and inaccuracy, reproducibility. 3.Analytic chemistry - qualitative, separation of inorganic ions into classes. Evidence of selected ions. 4.Basics of volumetric analysis: acidimetry, alcalimetry; Gravimetric analysis. 5.Comprehensive reaction and its application in laboratory analysis and volumetric methods; Precipitation reactions and their application in analytical chemistry. 6.Oxidation-reduction reaction and its application in a laboratory analysis. 7.Potentiometry, pH, ion selective electrode. 8. Principles of an organic analysis. 9.Spectrophotometry, flame photometry and atomic absorption spectrometry, infrared spectrometry. 10.Separation methods: Electrophoretic methods, chromatographic methods. 11.Mass spectrometry. 12.Immunochemical methods - immunoprecipitation, immunoanalysis. 13.Multiplex, chip technology, modern analytical approaches. Seminars: 1.-2. Patient´s preparation for harvest. Harvest systems. 3.-4. Calculations of parameters of quality in laboratory investigation. Preparation of control system in laboratory investigation. 5.-6. Application of flow cytometry (Multiplex,..). 7.-10.Laboratory practice - evidence of ions, volumetric determination. 11.-13. Laboratory practice - spectrum methods.

Learning activities and teaching methods
Lecture, Practicum
  • Contact hours - 39 hours per semester
  • Preparation for an examination (30-60) - 30 hours per semester
prerequisite
professional knowledge
No particular prerequisites specified.
learning outcomes
A student acquires proper laboratory skills and habits in analytic branch, general procedures in analysis of biological material. He/she is acquainted with laboratory equipment. He/she is able to calculate parameters of quality of laboratory investigation and principles of observation.
teaching methods
Lecture
Practicum
assessment methods
Combined exam
Skills demonstration during practicum
Recommended literature
  • Dvořáčková, S. a kolektiv. Praktikum lékařské chemie a biochemie. Univerzita Palackého, Olomouc 2006..
  • Jančářová, I. a Jančář, L. Analytická chemie, Mendelova zemědělská a lesnická univerzita, Brno 2003.
  • Křížek, M., Drbal K. Analytická chemie, Skriptum JU 1999.
  • Opekar, F. et al. Základní analytická chemie, Karolinum, Praha, 2003.
  • Štern, P., Kocna, P. Obecná a klinická biochemie pro bakalářské obory studia, Karolinum, Praha 2004.
  • Vondrák, D., Vulterin, J. Analytická. chemie, Praha: Státní nakladatelství technické literatury, 1985.
  • Waisser, K. Organická chemie I, Karolinum, Praha 2001.
  • Waisser, K. Organická chemie II., Karolinum, Praha 2000.


Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester